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验证数据展示

  • WB analysis using 85245-5-RR (same clone as 85245-5-PBS)

    WB analysis using 85245-5-RR (same clone as 85245-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85245-5-RR (WWTR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85245-5-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 85245-5-RR (same clone as 85245-5-PBS)

    WB analysis of A549 using 85245-5-RR (same clone as 85245-5-PBS)

    WB result of WWTR1/TAZ antibody (85245-5-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWTR1/TAZ transfected A549 cells. This data was developed using the same antibody clone with 85245-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85245-5-RR (same clone as 85245-5-PBS)

    IF Staining of HeLa using 85245-5-RR (same clone as 85245-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WWTR1/TAZ antibody (85245-5-RR, Clone: 252185D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85245-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85245-5-RR

    Affinity and Kinetic Characterization of 85245-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85245-5-RR against Human WWTR1/TAZ were performed. The affinity constant is 2.97 nM.

WWTR1/TAZ Recombinant monoclonal antibody, PBS Only

WWTR1/TAZ Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85245-5-PBS

产品说明书

CloneNo. 252185D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

TAZ, WWTR1, Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85245-5-PBS targets WWTR1/TAZ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg6470

Product name: Recombinant Human WWTR1/TAZ protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-400 aa of NM_001168278.2

Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 WW domain containing transcription regulator 1
别名 TAZ, WWTR1, Transcriptional coactivator with PDZ-binding motif, WW domain-containing transcription regulator protein 1
计算分子量44 kDa
观测分子量 50 kDa
GenBank蛋白编号NM_001168278.2
基因名称 WWTR1
Gene ID (NCBI) 25937
RRIDAB_3744121
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9GZV5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

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