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验证数据展示

  • WB analysis of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    WB analysis of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    IF Staining of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using WT1 antibody (12609-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • WB analysis using 12609-1-AP (same clone as 12609-1-PBS)

    WB analysis using 12609-1-AP (same clone as 12609-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    FC experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human WT1 (12609-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    IP experiment of K-562 using 12609-1-AP (same clone as 12609-1-PBS)

    IP result of anti-WT1 (IP:12609-1-AP, 4ug; Detection:12609-1-AP 1:1000) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 12609-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

WT1 Polyclonal antibody, PBS Only

WT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12609-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), IP, ELISA

别名

Wilms tumor 1, Wilms tumor protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12609-1-PBS targets WT1 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3337

Product name: Recombinant human WT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-302 aa of BC032861

Sequence: MEKGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRMHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Wilms tumor 1
别名 Wilms tumor 1, Wilms tumor protein
计算分子量 449 aa, 49 kDa, 57 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC032861
基因名称 WT1
Gene ID (NCBI) 7490
RRIDAB_2216225
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19544
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.