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验证数据展示

  • WB analysis of PC-3 using 12124-2-AP (same clone as 12124-2-PBS)

    WB analysis of PC-3 using 12124-2-AP (same clone as 12124-2-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12124-2-AP (WSB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

    IP experiment of mouse kidney using 12124-2-AP (same clone as 12124-2-PBS)

    IP result of anti-WSB2 (IP:12124-2-AP, 3ug; Detection:12124-2-AP 1:800) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 12124-2-AP (same clone as 12124-2-PBS)

    IF Staining of HEK-293 using 12124-2-AP (same clone as 12124-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using WSB2 antibody (12124-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12124-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WSB2 Polyclonal antibody, PBS Only

WSB2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 12124-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12124-2-PBS targets WSB2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2768

Product name: Recombinant human WSB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-404 aa of BC015887

Sequence: MEAGEEPLLLAELKPGRPHQFDWKSSCETWSVAFSPDGSWFAWSQGHCIVKLIPWPLEEQFIPKGFEAKSRSSKNETKGRGSPKEKTLDCGQIVWGLAFSPWPSPPSRKLWARHHPQVPDVSCLVLATGLNDGQIKIWEVQTGLLLLNLSGHQDVVRDLSFTPSGSLILVSASRDKTLRIWDLNKHGKQIQVLSGHLQWVYCCSISPDCSMLCSAAGEKSVFLWSMRSYTLIRKLEGHQSSVVSCDFSPDSALLVTASYDTNVIMWDPYTGERLRSLHHTQVDPAMDDSDVHISSLRSVCFSPEGLYLATVADDRLLRIWALELKTPIAFAPMTNGLCCTFFPHGGVIATGTRDGHVQFWTAPRVLSSLKHLCRKALRSFLTTYQVLALPIPKKMKEFLTYRTF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat and SOCS box-containing 2
别名
计算分子量 404 aa, 45 kDa
观测分子量 50 kDa
GenBank蛋白编号BC015887
基因名称 WSB2
Gene ID (NCBI) 55884
RRIDAB_2216206
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYS7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WSB2, also named SBA2, encodes WD repeat and SOCS box-containing protein 2. WSB2 may be a substrate-recognition component of an E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. WSB2 is a novel protein supposed to be involved in male- and female-specific gonadal development, and also a regulator of IL-21 receptor expression and IL21-induced signal transduction. WSB2 might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological functions need further studies.

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