验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, human kidney tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14761-1-AP targets WRAP53 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | WRAP53 fusion protein Ag6522 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat containing, antisense to TP53 |
| 别名 | TCAB1, WDR79, WRAP53 |
| 计算分子量 | 59 kDa |
| 观测分子量 | 72-75 kDa |
| GenBank蛋白编号 | BC002336 |
| 基因名称 | WRAP53 |
| Gene ID (NCBI) | 55135 |
| RRID | AB_1939806 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BUR4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WRAP53 antibody 14761-1-AP | Download protocol |
| IHC protocol for WRAP53 antibody 14761-1-AP | Download protocol |
| IP protocol for WRAP53 antibody 14761-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. | ||
Nat Chem Biol Observation of processive telomerase catalysis using high-resolution optical tweezers. | ||
Oncogenesis RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.
| ||
Aging (Albany NY) Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism. | ||
RNA Biol Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.
| ||
Cancer Cell Int Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells. |
