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验证数据展示

  • WB analysis using 27177-1-AP (same clone as 27177-1-PBS)

    WB analysis using 27177-1-AP (same clone as 27177-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 27177-1-AP (same clone as 27177-1-PBS)

    IP experiment of mouse kidney using 27177-1-AP (same clone as 27177-1-PBS)

    IP result of anti-WNT7A (IP:27177-1-AP, 4ug; Detection:27177-1-AP 1:2000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 27177-1-AP (same clone as 27177-1-PBS)

    IF Staining of HepG2 using 27177-1-AP (same clone as 27177-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (27177-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27177-1-PBS in a different storage buffer formulation.

WNT7A Polyclonal antibody, PBS Only

WNT7A Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 27177-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Protein Wnt-7a, Protein Wnt 7a

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27177-1-PBS targets WNT7A in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26060

Product name: Recombinant human WNT7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-305 aa of BC008811

Sequence: DCGCDKEKQGQYHRDEGWKWGGCSADIRYGIGFAKVFVDAREIKQNARTLMNLHNNEAGRKILEENMKLECKCHGVSGSCTTKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASRNKRPTFLKIKKPLSYRKPMDTDLVYIEKSPNYCEEDPVTGSVGTQGRACNKTAPQASGCD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 7A
别名 Protein Wnt-7a, Protein Wnt 7a
计算分子量 39 kDa
观测分子量 30-39 kDa
GenBank蛋白编号BC008811
基因名称 WNT7A
Gene ID (NCBI) 7476
RRIDAB_3085932
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00755
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WNT7A belongs to the Wnt family. WNT7A plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PMID: 16826533). WNT7A is required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PMID:30026314). WNT7A is highly expressed in basal cells and is secreted in abundance by basal cells (PMID: 36318668).

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