验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse liver tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10605-1-AP targets WNT7A/B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0874 Product name: Recombinant human WNT7A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC008811 Sequence: MNRKARRCLGHLFLSLGMVYLRIGGFSSVVALGASIICNKIPGLAPRQRAICQSRPDAIIVIGEGSQMGLDECQFQFRNGRWNCSALGERTVFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQGQYHRDEGWKWGGCSADIRYGIGFAKVFVDAREIKQNARTLMNLHNNEAGRKILEENMKLECKCHGVSGSCTTKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASRNKRPTFLKIKKPLSYRKPMDTDLVYIEKSPNYCEEDPVTGSVGTQGRACNKTAPQASGCDLMCCGRGYNTHQYARVWQCNCKFHWCCYVKCNTCSERTEMYTCK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | wingless-type MMTV integration site family, member 7A |
| 别名 | WNT7B, WNT7A, Protein Wnt 7a, Protein Wnt-7a |
| 计算分子量 | 39 kDa |
| 观测分子量 | 30-39 kDa |
| GenBank蛋白编号 | BC008811 |
| 基因名称 | WNT7A |
| Gene ID (NCBI) | 7476 |
| RRID | AB_2215625 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00755 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt7a is normally expressed in several organs, including the lung, testis, lymph node, and brain. As an oncogenic autocrine glycoprotein, Wnt7a promotes tumor invasion and distant metastasis with cancer-associated fibroblasts. Wnt7b is required for endothelial cells derived from pluripotent stem cells to acquire blood-brain barrier properties and may play an important role in the development of myopia in humans. This antibody can both recognize WNT7A and WNT7B.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for WNT7A/B antibody 10605-1-AP | Download protocol |
| IHC protocol for WNT7A/B antibody 10605-1-AP | Download protocol |
| IP protocol for WNT7A/B antibody 10605-1-AP | Download protocol |
| WB protocol for WNT7A/B antibody 10605-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Kidney Int Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis | ||
Autophagy WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. | ||
Nat Commun Spatiotemporal interplay between epithelial and mesenchymal cells drives human dentinogenesis. | ||
Cell Rep Med SIRT1 mediates brain metabolic and developmental consequences of methionine synthase deficiency in inborn errors of cobalamin metabolism. | ||
Sci Adv Modulation of blood-tumor barrier transcriptional programs improves intratumoral drug delivery and potentiates chemotherapy in GBM | ||
Free Radic Biol Med ZNF831-YTHDF1-DNMT1/3a feedback loop regulates lung carcinogenesis and progression through WNT7B-FZD5-β-catenin signalling axis |
