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验证数据展示

  • WB analysis of SGC-7901 using 24201-1-AP (same clone as 24201-1-PBS)

    WB analysis of SGC-7901 using 24201-1-AP (same clone as 24201-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 24201-1-AP (same clone as 24201-1-PBS)

    IHC staining of mouse brain using 24201-1-AP (same clone as 24201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 24201-1-AP (same clone as 24201-1-PBS)

    IHC staining of mouse brain using 24201-1-AP (same clone as 24201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24201-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WNT6 Polyclonal antibody, PBS Only

WNT6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24201-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24201-1-PBS targets WNT6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20889

Product name: Recombinant human WNT6 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 36-365 aa of BC004329

Sequence: DPTSICRKARRLAGRQAELCQAEPEVVAELARGARLGVRECQFQFRFRRWNCSSHSKAFGRILQQDIRETAFVFAITAAGASHAVTQACSMGELLQCGCQAPRGRAPPRPSGLPGTPGPPGPAGSPEGSAAWEWGGCGDDVDFGDEKSRLFMDARHKRGRGDIRALVQLHNNEAGRLAVRSHTRTECKCHGLSGSCALRTCWQKLPPFREVGARLLERFHGASRVMGTNDGKALLPAVRTLKPPGRADLLYAADSPDFCAPNRRTGSPGTRGRACNSSAPDLSGCDLLCCGRGHRQESVQLEENCLCRFHWCCVVQCHRCRVRKELSLCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 6
别名
计算分子量 39 kDa
观测分子量 42 kDa
GenBank蛋白编号BC004329
基因名称 WNT6
Gene ID (NCBI) 7475
RRIDAB_2879454
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6F9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WNT6 belongs to the Wnt family. It is ligand for members of the frizzled family of seven transmembrane receptors. WNT6 is likely to signal over only few cell diameters. WNT6 may be together with CAV1 to promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. And WNT6 is mainly expressed in brain, endometrium epithelium, gastric cancer cell lines and gastric cancer tissues (PMID: 22370641).

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