• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

WNT5A/B Polyclonal antibody

WNT5A/B Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 29793-1-AP

Print datasheet

Synonyms

hWNT5A, Protein Wnt 5a, WNT5A, WNT5A B

验证数据展示

  • WB analysis using 29793-1-AP

    WB analysis using 29793-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 29793-1-AP

    IF Staining of HeLa using 29793-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT5A antibody (29793-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, SKOV-3 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29793-1-AP targets WNT5A/B in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity Human
Cited Reactivityhuman, rat
Immunogen WNT5A/B fusion protein Ag31507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name wingless-type MMTV integration site family, member 5A
Synonyms hWNT5A, Protein Wnt 5a, WNT5A, WNT5A B
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC064694
Gene Symbol WNT5A
Gene ID (NCBI) 7474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides.

实验方案

Product Specific Protocols
WB protocol for WNT5A/B antibody 29793-1-APDownload protocol
IF protocol for WNT5A/B antibody 29793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

J Biol Chem

Exosomal miR-23a-3p derived from human umbilical cord mesenchymal stem cells promotes remyelination in central nervous system demyelinating diseases by targeting Tbr1/Wnt pathway

Authors - Danqing Qin
View Article
humanWB,IHC

J Orthop Res

Increased Wnt5a/ROR2 signaling is associated with chondrogenesis in meniscal degeneration

Authors - Yusuke Inoue
View Article