WNT4 Polyclonal antibody

WNT4 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14371-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

IHC, IF/ICC, ELISA and More (2)

WNT 4, UNQ426/PRO864, SERKAL, Protein Wnt-4, Protein Wnt 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14371-1-AP targets WNT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 WNT4 fusion protein Ag5741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 4
别名 WNT 4, UNQ426/PRO864, SERKAL, Protein Wnt-4, Protein Wnt 4
计算分子量 39 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC057781
基因名称 WNT4
Gene ID (NCBI) 54361
RRIDAB_2215428
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56705
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT4 belongs to the Wnt family. WNT4 is critical for embryonic organogenesis, but later plays a unique role among Wnt proteins in the development and maintenance of müllerian and reproductive tissues (PMID: 33963381). The mesenchymal stem cells can improve cardiac function after cardiac injury by regulating endothelial cells via the Wnt4/β-catenin signaling pathway (PMID: 20551912). WNT4 may be modified by glycosylation, leading to an increase in molecular weight.

实验方案

Product Specific Protocols
IHC protocol for WNT4 antibody 14371-1-APDownload protocol
IF protocol for WNT4 antibody 14371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny

Authors - Hua Du
humanWB

Endocrinology

PMID: 31748790

Authors - Alison M Neff
rat

Int J Clin Exp Pathol

Wnt4/β-catenin signaling pathway modulates balloon-injured carotid artery restenosis via disheveled-1.

Authors - Junyi Hua
mouseIHC

Neurochem Res

Wnt Signaling is Altered by Spinal Cord Neuronal Dysfunction in Amyotrophic Lateral Sclerosis Transgenic Mice.

Authors - Yu Li L
humanIF

Cell Death Dis

The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.

Authors - Nanchao Hong
mouseIHC

BMC Complement Med Ther

Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.

Authors - Bing Cao
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