验证数据展示
经过测试的应用
Positive WB detected in | fetal human brain tissue, MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 39 publications below |
产品信息
26744-1-AP targets WNT3A in WB, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | WNT3A fusion protein Ag25051 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | wingless-type MMTV integration site family, member 3A |
别名 | Protein Wnt 3a, Protein Wnt-3a |
计算分子量 | 352 aa, 39 kDa |
观测分子量 | 42 kDa |
GenBank蛋白编号 | BC103921 |
基因名称 | WNT3A |
Gene ID (NCBI) | 89780 |
RRID | AB_2918108 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P56704 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders. WNT3A is a secreted glycoprotein that is involved in both canonical and non-canonical Wnt signaling pathways. The canonical Wnt pathway, also known as the β-catenin-dependent pathway, regulates gene expression by stabilizing β-catenin, which then translocates to the nucleus and interacts with transcription factors like TCF/LEF. It's shown to promote trophectoderm formation in embryonic stem cells. It's reported that the canonical Wnt/β-catenin signaling pathway also contributes to the carcinogenesis and progression of lung cancer cell lines. The non-canonical pathways, which are β-catenin-independent, are involved in cytoskeletal organization and cell polarity.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for WNT3A antibody 26744-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Food Funct Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism | ||
Aging (Albany NY) Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats. | ||
Arch Toxicol Developmental exposure to nonylphenol induced rat axonal injury in vivo and in vitro. | ||
Apoptosis SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration. | ||
Biomed Pharmacother BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways. | ||
J Cancer Demethylzelasteral inhibits proliferation and EMT via repressing Wnt/β-catenin signaling in esophageal squamous cell carcinoma. |