验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 39 publications below |
产品信息
26744-1-AP targets WNT3A in WB, ELISA applications and shows reactivity with human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, Mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | WNT3A fusion protein Ag25051 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | wingless-type MMTV integration site family, member 3A |
| 别名 | Protein Wnt 3a, WNT3A |
| 计算分子量 | 352 aa, 39 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC103921 |
| 基因名称 | WNT3A |
| Gene ID (NCBI) | 89780 |
| RRID | AB_2918108 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56704 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WNT3A antibody 26744-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Food Funct Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism | ||
Aging (Albany NY) Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats. | ||
Arch Toxicol Developmental exposure to nonylphenol induced rat axonal injury in vivo and in vitro. | ||
Apoptosis SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration. | ||
Biomed Pharmacother BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways. | ||
J Cancer Demethylzelasteral inhibits proliferation and EMT via repressing Wnt/β-catenin signaling in esophageal squamous cell carcinoma. |
