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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28156-1-AP (same clone as 28156-1-PBS)

    WB analysis using 28156-1-AP (same clone as 28156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28156-1-AP (WNT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28156-1-AP (same clone as 28156-1-PBS)

    IHC staining of mouse brain using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

    IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

    IHC staining of mouse colon using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 28156-1-AP (same clone as 28156-1-PBS)

    IHC staining of mouse kidney using 28156-1-AP (same clone as 28156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28156-1-AP (WNT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28156-1-PBS in a different storage buffer formulation.

WNT3 Polyclonal antibody, PBS Only

WNT3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28156-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28156-1-PBS targets WNT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27965

Product name: Recombinant human WNT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-306 aa of BC114219

Sequence: SCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family, member 3
别名 INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3
计算分子量 355 aa, 40 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC114219
基因名称 WNT3
Gene ID (NCBI) 7473
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56703
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WNT3 is also named as INT4. WNT3 is required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis (PMID: 14872406). Wnt3 is up-regulated in cancer tissues, such as gastric cancer and lung cancer. Wnt3, as a member of Wnt family, has been proved to promote the stabilization of β-catenin to regulate the radiation sensitivity of cancer cells (PMID: 31851619).