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验证数据展示

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22326-1-AP (WNK4 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 22326-1-AP

    WB analysis using 22326-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22326-1-AP (WNK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 22326-1-AP

    IHC staining of human colon using 22326-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22326-1-AP (WNK4 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WNK4 Polyclonal antibody

WNK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22326-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, PRKWNK4, PHA2B, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, K-562 cells, PC-12 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

22326-1-AP targets WNK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag17773

Product name: Recombinant human WNK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 845-1243 aa of BC136664

Sequence: PISSQVSSNPSPHPTSSPLPFSSSTPEFPVPLSQCPWSSLPTTSPPTFSPTCSQVTLSSPFFPPCPSTSSFPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPSPLPSLPLPPPVAPGGQESPSPHTAEVESEASPPPARPLPGEARLAPISEEGKPQLVGRFQVTSSKEPAEPLPLQPTSPTLSGSPKPSTPQLTSESSDTEDSAGGGPETREALAESDRAAEGLGAGVEEEGDDGKEPQVGGSPQPLSHPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQSLRQKHLSEVETLQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WNK lysine deficient protein kinase 4
别名 Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, PRKWNK4, PHA2B, EC:2.7.11.1
计算分子量 1243 aa, 135 kDa
观测分子量 180 kDa
GenBank蛋白编号BC136664
基因名称 WNK4
Gene ID (NCBI) 65266
RRIDAB_2879072
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96J92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WNK4 antibody 22326-1-APDownload protocol
IHC protocol for WNK4 antibody 22326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.

Authors - Chee-Hong Chan
View Article
WB

Biochem J

p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.

Authors - Yutaro Mori
View Article
mouseWB

bioRxiv

DOPAMINE D4 RECEPTOR DOWN-REGULATES RENAL SODIUM CHLORIDE COTRANSPORTER VIA UBIQUITINATION-ASSOCIATED LYSOSOME DEGRADATION

Authors - Mingzhuo Zhang
View Article