- Featured Product
- KD/KO Validated
WNK1 Polyclonal antibody
WNK1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy WNK1 Ready-To-Use IHC Kit. WNK1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28357-1-AP targets WNK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | WNK1 fusion protein Ag28034 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | WNK lysine deficient protein kinase 1 |
| Synonyms | Erythrocyte 65 kDa protein, HSAN2, HSN2, hWNK1, KDP, KIAA0344, Kinase deficient protein, p65, PHA2C, PRKWNK1, PSK, WNK1 |
| Calculated Molecular Weight | 251 kDa |
| Observed Molecular Weight | 250-300 kDa |
| GenBank Accession Number | BC021121 |
| Gene Symbol | WNK1 |
| Gene ID (NCBI) | 65125 |
| RRID | AB_2881119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4A3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WNK1 antibody 28357-1-AP | Download protocol |
| IHC protocol for WNK1 antibody 28357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Carcinog Focal adhesion kinase confers lenvatinib resistance in hepatocellular carcinoma via the regulation of lysine-deficient kinase 1
| ||
Exp Cell Res Human adenylate kinase 6 regulates WNK1 (with no lysine kinase-1) phosphorylation states and affects ion homeostasis in NT2 cells. | ||
BMC Cancer Lymphocyte migration regulation related proteins in urine exosomes may serve as a potential biomarker for lung cancer diagnosis |
