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验证数据展示

  • IHC staining of mouse pancreas using 32378-1-AP

    IHC staining of mouse pancreas using 32378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32378-1-AP (WIZ antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 32378-1-AP

    IHC staining of mouse pancreas using 32378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32378-1-AP (WIZ antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 32378-1-AP

    IF Staining of U-251 using 32378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using WIZ antibody (32378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

WIZ Polyclonal antibody

WIZ Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 32378-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

Protein Wiz, Widely-interspaced zinc finger-containing protein, Zinc finger protein 803, ZNF803

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32378-1-AP targets WIZ in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37337

Product name: Recombinant human WIZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 530-745 aa of BC136641

Sequence: YLGSVAAKRPLQEDRLLPAEVKAKTYIQTELPFKAKTLHEKTSHSSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIKHRPQKVGAYRSYIQGGRPFTKKFRSAGHGRDSDKRPSLGLAPGGLAVVGRSAGGEPGPEAGRAADGGERPLAASPPGTVKAEEHQRQNINKFERRQARPPDASAARGGEDTNDLQQKLEEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 widely interspaced zinc finger motifs
别名 Protein Wiz, Widely-interspaced zinc finger-containing protein, Zinc finger protein 803, ZNF803
GenBank蛋白编号BC136641
基因名称 WIZ
Gene ID (NCBI) 58525
RRIDAB_3742588
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95785
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WIZ protein, whose full name is "Widely-interspaced zinc finger-containing protein," belongs to the Krueppel-type C2H2 zinc finger protein family. Its core function is to act as a chromatin-associated transcription factor that operates within the nucleus, where it links the histone methyltransferases EHMT1 and EHMT2 with the CTBP transcriptional corepressor complex, thereby participating in the regulation of gene transcription activity.

实验方案

Product Specific Protocols
IF protocol for WIZ antibody 32378-1-APDownload protocol
IHC protocol for WIZ antibody 32378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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