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验证数据展示

  • IHC staining of mouse pancreas using 32378-1-AP

    IHC staining of mouse pancreas using 32378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32378-1-AP (WIZ antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 32378-1-AP

    IHC staining of mouse pancreas using 32378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32378-1-AP (WIZ antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 32378-1-AP

    IF Staining of U-251 using 32378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using WIZ antibody (32378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

WIZ Polyclonal antibody

WIZ Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 32378-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

Protein Wiz, Widely-interspaced zinc finger-containing protein, Zinc finger protein 803, ZNF803

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32378-1-AP targets WIZ in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37337

Product name: Recombinant human WIZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 530-745 aa of BC136641

Sequence: YLGSVAAKRPLQEDRLLPAEVKAKTYIQTELPFKAKTLHEKTSHSSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIKHRPQKVGAYRSYIQGGRPFTKKFRSAGHGRDSDKRPSLGLAPGGLAVVGRSAGGEPGPEAGRAADGGERPLAASPPGTVKAEEHQRQNINKFERRQARPPDASAARGGEDTNDLQQKLEEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 widely interspaced zinc finger motifs
别名 Protein Wiz, Widely-interspaced zinc finger-containing protein, Zinc finger protein 803, ZNF803
GenBank蛋白编号BC136641
基因名称 WIZ
Gene ID (NCBI) 58525
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95785
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for WIZ antibody 32378-1-APDownload protocol
IHC protocol for WIZ antibody 32378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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