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验证数据展示

  • WB analysis of Jurkat using 55119-1-AP

    WB analysis of Jurkat using 55119-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WEE2 Polyclonal antibody

WEE2 Polyclonal Antibody for WB, ELISA
Cat No. 55119-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

WEE1 homolog 2 (S. pombe), Wee1 like protein kinase 1B, Wee1 like protein kinase 2, WEE1B, Wee1B kinase, WEE2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, human ovary tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55119-1-AP targets WEE2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WEE1 homolog 2 (S. pombe)
别名 WEE1 homolog 2 (S. pombe), Wee1 like protein kinase 1B, Wee1 like protein kinase 2, WEE1B, Wee1B kinase, WEE2
计算分子量 63 kDa
观测分子量 63-66 kDa
GenBank蛋白编号NM_001105558
基因名称 WEE2
Gene ID (NCBI) 494551
RRIDAB_10951857
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0C1S8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Taking the long double-stranded RNA-mediated RNA interference approach, down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. Overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.(PMID: 20200212) The antibody is specific to WEE2.

实验方案

Product Specific Protocols
WB protocol for WEE2 antibody 55119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols