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验证数据展示

  • WB analysis using 25540-1-AP

    WB analysis using 25540-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25540-1-AP (WDR92 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25540-1-AP

    IHC staining of human testis using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon using 25540-1-AP

    IHC staining of human colon using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25540-1-AP (WDR92 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 25540-1-AP

    IF Staining of HepG2 using 25540-1-AP

    Immunofluorescent analysis of HepG2 cells using 25540-1-AP (WDR92 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 25540-1-AP

    IHC staining of human colon using 25540-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 25540-1-AP (WDR92 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WDR92 Polyclonal antibody

WDR92 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25540-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

WD repeat-containing protein Monad, WD repeat-containing protein 92, WD repeat domain 92, Dynein axonemal assembly factor 10, DNAAF10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25540-1-AP targets WDR92 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22181

Product name: Recombinant human WDR92 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-357 aa of BC066657

Sequence: MSAFEKPQIIAHIQKGFNYTVFDCKWVPCSAKFVTMGNFARGTGVIQLYEIQHGDLKLLREIEKAKPIKCGTFGATSLQQRYLATGDFGGNLHIWNLEAPEMPVYSVKGHKEIINAIDGIGGLGIGEGAPEIVTGSRDGTVKVWDPRQKDDPVANMEPVQGENKRDCWTVAFGNAYNQEERVVCAGYDNGDIKLFDLRNMALRWETNIKNGVCSLEFDRKDISMNKLVATSLEGKFHVFDMRTQHPTKGFASVSEKAHKSTVWQVRHLPQNRELFLTAGGAGGLHLWKYEYPIQRSKKDSEGIEMGVAGSVSLLQNVTLSTQPISSLDWSPDKRGLCVCSSFDQTVRVLIVTKLNKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 92
别名 WD repeat-containing protein Monad, WD repeat-containing protein 92, WD repeat domain 92, Dynein axonemal assembly factor 10, DNAAF10
计算分子量 357 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC066657
基因名称 WDR92
Gene ID (NCBI) 116143
RRIDAB_2880124
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MX6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for WDR92 antibody 25540-1-APDownload protocol
IHC protocol for WDR92 antibody 25540-1-APDownload protocol
WB protocol for WDR92 antibody 25540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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