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验证数据展示

  • WB analysis of HL-60 using 21354-1-AP

    WB analysis of HL-60 using 21354-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21354-1-AP (WDR82 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21354-1-AP

    IHC staining of mouse brain using 21354-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21354-1-AP (WDR82 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21354-1-AP

    IHC staining of mouse brain using 21354-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21354-1-AP (WDR82 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 21354-1-AP

    WB analysis using 21354-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21354-1-AP (WDR82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WDR82 Polyclonal antibody

WDR82 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21354-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WD repeat domain 82, WDR82, WDR82A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells; K-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21354-1-AP targets WDR82 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag15762

Product name: Recombinant human WDR82 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 175-313 aa of BC018941

Sequence: DLRSFDKGPFATFKMQYDRTCEWTGLKFSNDGKLILISTNGSFIRLIDAFKGVVMHTFGGYANSKAVTLEASFTPDSQFIMIGSEDGKIHVWNGESGIKVAVLDGKHTGPITCLQFNPKFMTFASACSNMAFWLPTIDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 82
别名 MST107, MSTP107, PRO2730, PRO34047, SWD2, TMEM113, WD repeat domain 82, WDR82, WDR82A
计算分子量 313 aa, 35 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC018941
基因名称 WDR82
Gene ID (NCBI) 80335
RRIDAB_3085654
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WDR82 antibody 21354-1-APDownload protocol
IHC protocol for WDR82 antibody 21354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency

Authors - Chikako Okubo
View Article
humanWB

J Exp Clin Cancer Res

Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer

Authors - Macarena Morillo-Huesca
View Article