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验证数据展示

  • WB analysis using 24874-1-AP (same clone as 24874-1-PBS)

    WB analysis using 24874-1-AP (same clone as 24874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24874-1-AP (WDR81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WDR81 antibody included
  • IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    IHC staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24874-1-AP (WDR81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WDR81 antibody included
  • IF Staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    IF Staining of mouse cerebellum using 24874-1-AP (same clone as 24874-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using WDR81 antibody (24874-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 24874-1-AP (same clone as 24874-1-PBS)

    IF Staining of HepG2 using 24874-1-AP (same clone as 24874-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WDR81 antibody (24874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24874-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WDR81 Polyclonal antibody, PBS Only

WDR81 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 24874-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

WD repeat-containing protein 81

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy WDR81 Ready-To-Use IHC Kit. WDR81 primary antibody included.

产品信息

24874-1-PBS targets WDR81 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21666

Product name: Recombinant human WDR81 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 323-692 aa of BC114568

Sequence: NDSRPENPGPLGPISGVGGGGLGSGSDDNALKQELPRSVHGLSGNWLAYWQYEIGVSQQDAHFHFHQIRLQSFPGHSGAVKCVAPLSSEDFFLSGSKDRTVRLWPLYNYGDGTSETAPRLVYTQHRKSVFFVGQLEAPQHVVSCDGAVHVWDPFTGKTLRTVEPLDSRVPLTAVAVMPAPHTSITMASSDSTLRFVDCRKPGLQHEFRLGGGLNPGLVRALAISPSGRSVVAGFSSGFMVLLDTRTGLVLRGWPAHEGDILQIKAVEGSVLVSSSSDHSLTVWKELEQKPTHHYKSASDPIHTFDLYGSEVVTGTVSNKIGVCSLLEPPSQATTKLSSENFRGTLTSLALLPTKRHLLLGSDNGVIRLLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 81
别名 WD repeat-containing protein 81
计算分子量 1941 aa, 212 kDa
观测分子量74-80 kDa, 250 kDa
GenBank蛋白编号BC114568
基因名称 WDR81
Gene ID (NCBI) 124997
RRIDAB_2879771
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ562E7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WD repeat-containing protein 81 (WDR81) is a 1941 amino acid protein, which contains one BEACH domain and five WD repeats. WDR81 is widely expressed in the brain. WDR81 has an association with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 2. WDR81 exists as six isoforms.

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