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验证数据展示

  • WB analysis using 86361-2-RR

    WB analysis using 86361-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86361-2-RR (WDR74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86361-2-RR

    IF Staining of HeLa using 86361-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR74 antibody (86361-2-RR, Clone: 251046A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86361-2-RR

    Affinity and Kinetic Characterization of 86361-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86361-2-RR against Human WDR74 were performed. The affinity constant is 1.02 nM.

WDR74 Recombinant monoclonal antibody

WDR74 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86361-2-RR

产品说明书

CloneNo. 251046A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

NSA1, WD repeat domain 74, WD repeat-containing protein 74

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MDA-MB-231 cells, A-673 cells, A375 cells, IMR-32 cells, RAW264.7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86361-2-RR targets WDR74 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14617

Product name: Recombinant human WDR74 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC006351

Sequence: MAAAAARWNHVWVGTETGILKGVNLQRKQAANFTAGGQPRREEAVSALCWGTGGETQMLVGCADRTVKHFSTEDGIFQGQRHCPGGEGMFRGLAQADGTLITCVDSGILRVWHDKDKDTSSDPLLELRVGPGVCRMRQDPAHPHVVATGGKENALKIWDLQGSEEPVFRAKNVRNDWLDLRVPIWDQDIQFLPGSQKLVTCTGYHQVRVYDPASPQRRPVLETTYGEYPLTAMTLTPGGNSVIVGNTHGQLAEIDLRQGRLLGCLKGLAGSVRGLQCHPSKPLLASCGLDRVLRIHRIQN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 WD repeat domain 74
别名 NSA1, WD repeat domain 74, WD repeat-containing protein 74
计算分子量 385 aa, 42 kDa
观测分子量40 kDa
GenBank蛋白编号BC006351
基因名称 WDR74
Gene ID (NCBI) 54663
RRIDAB_3744838
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6RFH5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers.

实验方案

Product Specific Protocols
IF protocol for WDR74 antibody 86361-2-RRDownload protocol
WB protocol for WDR74 antibody 86361-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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