验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, MG-63 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33145-1-AP targets WDR70 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag27019 Product name: Recombinant human WDR70 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 393-649 aa of BC009648 Sequence: LASRGGDDSLKLWDIRQFNKPLFSASGLPTMFPMTDCCFSPDDKLIVTGTSIQRGCGSGKLVFFERRTFQRVYEIDITDASVVRCLWHPKLNQIMVGTGNGLAKVYYDPNKSQRGAKLCVVKTQRKAKQAETLTQDYIITPHALPMFREPRQRSTRKQLEKDRLDPLKSHKPEPPVAGPGRGGRVGTHGGTLSSYIVKNIALDKTDDSNPREAILRHAKAAEDSPYWVSPAYSKTQPKTMFAQVESDDEEAKNEPEW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 70 |
| 别名 | WD repeat-containing protein 70 |
| 计算分子量 | 654 aa, 73 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC009648 |
| 基因名称 | WDR70 |
| Gene ID (NCBI) | 55100 |
| RRID | AB_3742870 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NW82 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WD repeat-containing protein 70 (WDR70), which is composed of six WD40 repeat domain functions as a subunit of CRL4 complex in homology-directed repair (HDR) by regulating the transcription of BRCA1, BRCA2, RAD51, and substantial additional transcriptomic changes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR70 antibody 33145-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
