验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, MDA-MB-231 cells, MDA-MB-453 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33624-1-AP targets WDR66 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36992 Product name: Recombinant human WDR66 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC028421 Sequence: MSDAAEAPREATGENGETEMKEEEEPNPNYKEVEDPQQESKDDTIAWRESQEEERKTGEEEGEEEEKEEEGKEDKKIVMEETEEKAGEVQEKEASGIQEETTVEPQEVTASMIRLETQITDSQSITSGIFPKTQRGSKSKLSLQLEDAETDELLRDLSTQIEFLDLDQISPEEQQISSPERQPSGELEEKTDRMPQDELGQERRDLEPENREEGQERRVSDIQSKAGISRESLVSSTTEDILFQKDKSTPVYPLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 66 |
| 别名 | CFAP251, Cilia- and flagella-associated protein 251, WD repeat-containing protein 66 |
| 观测分子量 | 46 kDa, 130kDa |
| GenBank蛋白编号 | BC028421 |
| 基因名称 | WDR66 |
| Gene ID (NCBI) | 144406 |
| RRID | AB_3743032 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8TBY9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR66 (WD repeat domain 66), also designated as CFAP251 (cilia- and flagella-associated protein 251), is a member of the WD40 repeat domain protein family. Members of this family typically serve as scaffold molecules facilitating the assembly of diverse protein complexes. The primary function of WDR66/CFAP251 is intimately associated with the structural integrity and motility of sperm flagella and motile cilia. As an essential constituent of the calmodulin- and spoke-associated complex, WDR66/CFAP251 localizes to the base of the axonemal radial spokes and plays a critical role in the proper assembly, stability, and motility of sperm flagella.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR66 antibody 33624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
