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  • Featured Product
  • KD/KO Validated

WDR48 Polyclonal antibody

WDR48 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16503-1-AP

Print datasheet

Synonyms

KIAA1449, P80, UAF1, USP1 associated factor 1, WD repeat domain 48, WD repeat endosomal protein, WDR48

验证数据展示

  • WB analysis using 16503-1-AP

    WB analysis using 16503-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16503-1-AP (WDR48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 16503-1-AP

    IP experiment of HEK-293 using 16503-1-AP

    IP result of anti-WDR48 (IP:16503-1-AP, 3ug; Detection:16503-1-AP 1:500) with HEK-293 cells lysate 3000ug.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, mouse heart tissue
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16503-1-AP targets WDR48 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen WDR48 fusion protein Ag9674 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 48
Synonyms KIAA1449, P80, UAF1, USP1 associated factor 1, WD repeat domain 48, WD repeat endosomal protein, WDR48
Calculated Molecular Weight502aa,57 kDa; 677aa,76 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC037168
Gene Symbol WDR48
Gene ID (NCBI) 57599
RRIDAB_2878266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAF3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for WDR48 antibody 16503-1-APDownload protocol
IP protocol for WDR48 antibody 16503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IP

Nat Commun

The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling

Authors - Victoria H Ng
View Article
humanWB

Cell Prolif

Comprehensive analysis of ubiquitin-specific protease 1 reveals its importance in hepatocellular carcinoma.

Authors - Yalei Zhao
View Article
humanWB

Cell Cycle

USP1-WDR48 deubiquitinase complex enhances TGF-β induced epithelial-mesenchymal transition of TNBC cells via stabilizing TAK1.

Authors - Dianwen Han
  • KD Validated
View Article
humanWB

J Biol Chem

The WD40-repeat protein WDR-48 promotes the stability of the deubiquitinating enzyme USP-46 by inhibiting its ubiquitination and degradation.

Authors - Molly Hodul
  • KD Validated
View Article
humanWB

Microbiol Spectr

USP1-Associated Factor 1 Modulates Japanese Encephalitis Virus Replication by Governing Autophagy and Interferon-Stimulated Genes

Authors - Jinchao Xing
  • KD Validated
View Article
mouseWB

iScience

The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis

Authors - Ziqi Wang
View Article