验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | Jurkat cells |
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 3 publications below |
产品信息
15110-1-AP targets WDR46 in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | WDR46 fusion protein Ag7095 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 46 |
| 别名 | WD repeat-containing protein BING4, WD repeat-containing protein 46, WD repeat domain 46, FP221, C6orf11 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC000388 |
| 基因名称 | WDR46 |
| Gene ID (NCBI) | 9277 |
| RRID | AB_2215408 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15213 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR46 antibody 15110-1-AP | Download protocol |
| IP protocol for WDR46 antibody 15110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Role of the small subunit processome in the maintenance of pluripotent stem cells. | ||
Biochem Cell Biol Dynamics of WD-repeat containing proteins in SSU processome components. | ||
Genes Cells Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21. | ||
Biochem Biophys Res Commun Nuclear localization of DMP1 proteins suggests a role in intracellular signaling. |
