验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19194-1-AP targets WDR45/WIPI4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | WDR45/WIPI4 fusion protein Ag6741 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 45 |
| 别名 | WDR45, WDRX1, WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat domain 45 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC000464 |
| 基因名称 | WDR45 |
| Gene ID (NCBI) | 11152 |
| RRID | AB_2215404 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y484 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR45/WIPI4 antibody 19194-1-AP | Download protocol |
| IHC protocol for WDR45/WIPI4 antibody 19194-1-AP | Download protocol |
| IF protocol for WDR45/WIPI4 antibody 19194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Sci Adv Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism.
| ||
Int J Mol Sci Iron Accumulation and Changes in Cellular Organelles in WDR45 Mutant Fibroblasts.
| ||
J Neurochem A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation
| ||
Am J Med Genet A Autophagic defects observed in fibroblasts from a patient with β-propeller protein-associated neurodegeneration. | ||
Eur J Med Genet Early onset developmental delay and epilepsy in pediatric patients with WDR45 variants. |
