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验证数据展示

  • WB analysis of HepG2 using 22614-1-AP (same clone as 22614-1-PBS)

    WB analysis of HepG2 using 22614-1-AP (same clone as 22614-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22614-1-AP (WDR33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22614-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 22614-1-AP (same clone as 22614-1-PBS)

    IP experiment of HeLa using 22614-1-AP (same clone as 22614-1-PBS)

    IP result of anti-WDR33 (IP:22614-1-AP, 4ug; Detection:22614-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 22614-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 22614-1-AP (same clone as 22614-1-PBS)

    IHC staining of human ovarian cancer using 22614-1-AP (same clone as 22614-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 22614-1-AP (WDR33 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22614-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WDR33 antibody included
  • IF Staining of HeLa using 22614-1-AP (same clone as 22614-1-PBS)

    IF Staining of HeLa using 22614-1-AP (same clone as 22614-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR33 antibody (22614-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 22614-1-PBS in a different storage buffer formulation.

WDR33 Polyclonal antibody, PBS Only

WDR33 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22614-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

pre-mRNA 3' end processing protein WDR33, WD repeat-containing protein 33, WD repeat-containing protein of 146 kDa, WDR 33

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy WDR33 Ready-To-Use IHC Kit. WDR33 primary antibody included.

产品信息

22614-1-PBS targets WDR33 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18382

Product name: Recombinant human WDR33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-239 aa of BC010283

Sequence: GRREESFRRGAPPRHEGRAPPRGRDGFPGPEDFGPEENFDASEEAARGRDLRGRGRGTPRGGRKGLLPTPDEFPRFEGGRKPDSWDGNREPGPGHEHFRDTPRPDHPPHDGHSPASRERSSSLQGMDMASLPPRKRPWHDGPGTSEHREMEAPGGPSEDRGGKGRGGPGPAQRVPKSGRSSSLDGEHHDGYHRDEPFGGPPGSGTPSRGGRSGSNWGRGSNMNSGPPRRGASRGGGRGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 33
别名 pre-mRNA 3' end processing protein WDR33, WD repeat-containing protein 33, WD repeat-containing protein of 146 kDa, WDR 33
计算分子量 1336 aa, 146 kDa
观测分子量 170 kDa
GenBank蛋白编号BC010283
基因名称 WDR33
Gene ID (NCBI) 55339
RRIDAB_2879135
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9C0J8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WD repeat domain 33 (WDR33), also named WDC146, is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination.This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is highly expressed in testis and the protein is localized to the nucleus. This gene may play important roles in the mechanisms of cytodifferentiation and/or DNA recombination. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. This antibody recognizes the full length WDR33 protein.

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