验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83802-4-RR targets WDR21C in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | WDR21C fusion protein Ag30748 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 21C |
| 别名 | WD repeat-containing protein 21C, WD repeat domain 21C, DDB1- and CUL4-associated factor 4-like protein 2, DCAF4L2, 240402H3 |
| 计算分子量 | 395 aa, 44 kDa |
| 观测分子量 | 44-50 kDa |
| GenBank蛋白编号 | BC111778 |
| 基因名称 | WDR21C |
| Gene ID (NCBI) | 138009 |
| RRID | AB_3671390 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8NA75 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR21C (WD repeat-containing protein 21C), also known as DCAF4L2. It is expected to be located in the cytoplasm, which is enriched in testis. WDR21C is a member of WD-repeat proteins, which commonly serve as mediators of protein-protein interplay. It is reported that elevated WDR21C expression in human colorectal cancer (CRC) significantly correlated with a more advanced clinical stage as in lymphatic and distant metastasis. More importantly, elevated WDR21C expression is an independent prognosis factor for survival (PMID: 27158335). The molecular weight of WDR21C is 43 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR21C antibody 83802-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |