验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human lung tissue, human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15165-1-AP targets WDR18 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag7282 Product name: Recombinant human WDR18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC001648 Sequence: MAAPMEVAVCTDSAAPMWSCIVWELHSGANLLTYRGGQAGPRGLALLNGEYLLAAQLGKNYISAWELQRKDQLQQKIMCPGPVTCLTASPNGLYVLAGVAESIHLWEVSTGNLLVILSRHYQDVSCLQFTGDSSHFISGGKDCLVLVWSLCSVLQADPSRIPAPRHVWSHHTLPITDLHCGFGGPLARVATSSLDQTVKLWEVSSGELLLSVLFDVSIMAVTMDLAEHHMFCGGSEGSIFQVDLFTWPGQRERSFHPEQDAGKVFKGHRNQVTCLSVSTDGSVLLSGSHDETVRLWDVQSKQCIRT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 18 |
| 别名 | R32184_1, WD repeat domain 18, WDR18 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC001648 |
| 基因名称 | WDR18 |
| Gene ID (NCBI) | 57418 |
| RRID | AB_2288469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BV38 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR18 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit. This complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation (PMID:21326211).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR18 antibody 15165-1-AP | Download protocol |
| IHC protocol for WDR18 antibody 15165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.
| ||
Mol Cell Proteomics Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. | ||
Mol Oncol PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis |
