验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33831-1-AP targets WDR12 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38016 Product name: Recombinant human WDR12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-423 aa of BC008082 Sequence: NISSEEVVEIEYVEKYTAPQPEQCMFHDDWISSIKGAEEWILTGSYDKTSRIWSLEGKSIMTIVGHTDVVKDVAWVKKDSLSCLLLSASMDQTILLWEWNVERNKVKALHCCRGHAGSVDSIAVDGSGTKFCSGSWDKMLKIWSTVPTDEEDEMEESTNRPRKKQKTEQLGLTRTPIVTLSGHMEAVSSVLWSDAEEICSASWDHTIRVWDVESGSLKSTLTGNKVFNCISYSPLCKRLASGSTDRHIRLWDPRTKDGSLVSLSLTSHTGWVTSVKWSPTHEQQLISGSLDNIVKLWDTRSCKAPLYDLAAHEDKVLSVDWTDTGLLLSGGADNKLYSYRYSPTTSHVGA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 12 |
| 别名 | Ribosome biogenesis protein WDR12, WD repeat-containing protein 12 |
| 计算分子量 | 423 aa, 48 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC008082 |
| 基因名称 | WDR12 |
| Gene ID (NCBI) | 55759 |
| RRID | AB_3743085 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9GZL7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR12 is a nucleolar WD-repeat scaffolding protein and an essential component of the PeBoW complex required for 60S ribosomal subunit maturation. By driving pre-rRNA processing, WDR12 supports cell growth and proliferation, while its disruption activates the p53-dependent nucleolar stress pathway. Upregulated in multiple cancers and genetically linked to cardiometabolic traits, WDR12 serves as a central regulator of ribosome biogenesis, cell-cycle control, and cellular homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR12 antibody 33831-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
