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验证数据展示

  • IHC staining of rat brain using 55009-1-AP

    IHC staining of rat brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55009-1-AP

    IHC staining of mouse brain using 55009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

WDFY3/ALFY Polyclonal antibody

WDFY3/ALFY Polyclonal Antibody for IHC, ELISA
Cat No. 55009-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA and More (2)

别名

WDFY3, WDFY3 / ALFY, Alfy, Autophagy linked FYVE protein, Autophagy-linked FYVE protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat brain tissue, mouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55009-1-AP targets WDFY3/ALFY in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat and FYVE domain containing 3
别名 WDFY3, WDFY3 / ALFY, Alfy, Autophagy linked FYVE protein, Autophagy-linked FYVE protein
计算分子量 395 kDa
GenBank蛋白编号NM_014991
基因名称 WDFY3
Gene ID (NCBI) 23001
RRIDAB_10863126
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.

实验方案

Product Specific Protocols
IHC protocol for WDFY3/ALFY antibody 55009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Cardiovasc Res

WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.

Authors - Shasha Zhang
  • KO Validated
View Article
mouseIHC,IF

In Vitro Cell Dev Biol Anim

Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS).

Authors - Huihui Han
View Article
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