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验证数据展示

  • WB analysis using 32283-1-AP (same clone as 32283-1-PBS)

    WB analysis using 32283-1-AP (same clone as 32283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32283-1-AP (WBP4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32283-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 32283-1-AP (same clone as 32283-1-PBS)

    IHC staining of mouse testis using 32283-1-AP (same clone as 32283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32283-1-AP (WBP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32283-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 32283-1-AP (same clone as 32283-1-PBS)

    IF Staining of U-251 using 32283-1-AP (same clone as 32283-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using WBP4 antibody (32283-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 32283-1-PBS in a different storage buffer formulation.

WBP4 Polyclonal antibody, PBS Only

WBP4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32283-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FBP21, FNBP21, Formin-binding protein 21, WBP-4, WW domain-binding protein 4

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32283-1-PBS targets WBP4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35400

Product name: Recombinant human WBP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of BC108310

Sequence: MADYWKSQPKKFCDYCKCWIADNRPSVEFHERGKNHKENVAKRISEIKQKSLDKAKEEEKASKEFAAMEAAALKAYQEDLKRLGLESEILEPSITPVTSTIPPTSTSNQQKEKKEKKKRKKDPSKGRWVEGITSEGYHYYYDLISGASQW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WW domain binding protein 4 (formin binding protein 21)
别名 FBP21, FNBP21, Formin-binding protein 21, WBP-4, WW domain-binding protein 4
观测分子量50-55 kDa
GenBank蛋白编号BC108310
基因名称 WBP4
Gene ID (NCBI) 11193
RRIDAB_3742568
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO75554
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WW domain-binding protein 4 (WBP4), formerly known as FBP21, is a 376-amino acid spliceosomal protein containing a zinc finger motif and two tandem WW domains. It is a member of the WW domain-containing protein family, which plays a role in various cellular processes including signal transduction, transcriptional regulation and protein degradation. The protein is characterised by its WW domains, which mediate interactions with proline-rich motifs in other proteins. WBP4 is involved in key signalling pathways such as Wnt and Notch and interacts with transcription factors and components of the ubiquitin-proteasome system.

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