验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10316-1-AP targets WBP11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | WBP11 fusion protein Ag0366 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | WW domain binding protein 11 |
别名 | SNP70, SIPP1, SH3 domain-binding protein SNP70, FAP90, CFAP90 |
计算分子量 | 70 kDa |
观测分子量 | 66-70 kDa |
GenBank蛋白编号 | BC001621 |
基因名称 | WBP11 |
Gene ID (NCBI) | 51729 |
RRID | AB_10694664 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y2W2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WBP11 (WW domain-binding protein 11) is a protein that in humans, is encoded by the WBP11 gene. The function of WBP11 is to play a role in the regulation of pre-mRNA processing. More specifically, this nuclear protein, colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. The predicted MW of this protein is 70 kDa, and this antibody specially recognises the 70 kDa protein.
实验方案
Product Specific Protocols | |
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WB protocol for WBP11 antibody 10316-1-AP | Download protocol |
IHC protocol for WBP11 antibody 10316-1-AP | Download protocol |
IF protocol for WBP11 antibody 10316-1-AP | Download protocol |
IP protocol for WBP11 antibody 10316-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |