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验证数据展示

  • WB analysis of mouse brain using 19755-1-AP

    WB analysis of mouse brain using 19755-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19755-1-AP

    IF Staining of HepG2 using 19755-1-AP

    Immunofluorescent analysis of HepG2 cells, using WASF4 antibody 19755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

WASF4-Specific Polyclonal antibody

WASF4-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19755-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

SCAR2, WASF4, WAS protein family, member 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19755-1-AP targets WASF4-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAS protein family, member 4
别名 SCAR2, WASF4, WAS protein family, member 4
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号Q8IV90
基因名称 WASF4
Gene ID (NCBI) 644739
RRIDAB_10646483
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASF4, also named as SCAR2, belongs to the SCAR/WAVE family. WASF4 is a downstream effector molecule which involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. The antibody is specific to WASF4.

实验方案

Product Specific Protocols
WB protocol for WASF4-Specific antibody 19755-1-APDownload protocol
IF protocol for WASF4-Specific antibody 19755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols