• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32584-1-AP

    WB analysis using 32584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32584-1-AP (WASF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 32584-1-AP

    IP experiment of HEK-293 using 32584-1-AP

    IP result of anti-WASF1 (IP:32584-1-AP, 4ug; Detection:32584-1-AP 1:3000) with HEK-293 cells lysate 1800 ug.

WASF1 Polyclonal antibody

WASF1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32584-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

Actin-binding protein WASF1, KIAA0269, Protein WAVE-1, SCAR1, Verprolin homology domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32584-1-AP targets WASF1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 WASF1 fusion protein Ag38505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAS protein family, member 1
别名 Actin-binding protein WASF1, KIAA0269, Protein WAVE-1, SCAR1, Verprolin homology domain-containing protein 1
计算分子量62kDa，559aa
观测分子量75 kDa
GenBank蛋白编号NM_003931.2
基因名称 WASF1
Gene ID (NCBI) 8936
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ92558
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WASF1 (Wiskott-Aldrich Syndrome Protein Family Member 1) is a gene encoding an actin-binding protein that belongs to the Wiskott-Aldrich syndrome protein family. It is primarily involved in regulating the reorganization of the actin cytoskeleton in cells, and is essential for processes such as cell morphology, motility, and signaling. WASF1 promotes actin polymerization by interacting with the Arp2/3 complex, thereby affecting cell deformation and migration. WASF1 is expressed in a variety of cell types, including tissues such as brain and testis. Its aberrant expression is associated with a variety of diseases, such as tumor invasion and metastasis.

实验方案

Product Specific Protocols
WB protocol for WASF1 antibody 32584-1-APDownload protocol
IP protocol for WASF1 antibody 32584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols