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WASP Polyclonal antibody

WASP Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10987-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells, mouse spleen tissue, Jurkat cells, Raji cells
Positive IP detected inRaji cells, rat spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy WAS Ready-To-Use IHC Kit. WAS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10987-1-AP targets WASP in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 WASP fusion protein Ag1444 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
别名 Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS
计算分子量 53 kDa
观测分子量 55-62 kDa
GenBank蛋白编号BC012738
基因名称 WASP
Gene ID (NCBI) 7454
RRIDAB_2304546
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42768
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.

实验方案

Product Specific Protocols
WB protocol for WASP antibody 10987-1-APDownload protocol
IHC protocol for WASP antibody 10987-1-APDownload protocol
IP protocol for WASP antibody 10987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Allergy Clin Immunol

Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.

Authors - Yukai Jing
human

Nat Commun

WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage.

Authors - Seong-Su Han
humanWB

J Exp Clin Cancer Res

miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.

Authors - Yongwen Li
  • KD Validated
humanWB

J Exp Clin Cancer Res

CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.

Authors - Dong-Fang Meng
humanWB,RIP,IP

Mol Cancer Res

LINC00869 Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation

Authors - Xiaowen Shao
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