验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, K-562 cells, mouse spleen tissue, Jurkat cells, Raji cells |
| Positive IP detected in | Raji cells, rat spleen tissue |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10987-1-AP targets WASP in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1444 Product name: Recombinant human WAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-163 aa of BC012738 Sequence: MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Wiskott-Aldrich syndrome (eczema-thrombocytopenia) |
| 别名 | Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS |
| 计算分子量 | 53 kDa |
| 观测分子量 | 55-62 kDa |
| GenBank蛋白编号 | BC012738 |
| 基因名称 | WASP |
| Gene ID (NCBI) | 7454 |
| RRID | AB_2304546 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42768 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for WASP antibody 10987-1-AP | Download protocol |
| IP protocol for WASP antibody 10987-1-AP | Download protocol |
| WB protocol for WASP antibody 10987-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Allergy Clin Immunol Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. | ||
Nat Commun WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage. | ||
J Exp Clin Cancer Res miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.
| ||
J Exp Clin Cancer Res CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. | ||
Mol Cancer Res LINC00869 Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation |
