验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20519-1-AP targets WAC in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag14355 Product name: Recombinant human WAC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 307-649 aa of BC004258 Sequence: ESTSGDKPVSHSCTTPSTSSASGLNPTSAPPTSASAVPVSPVPQSPIPPLLQDPNLLRQLLPALQATLQLNNSNVDISKINEVLTAAVTQASLQSIIHKFLTAGPSAFNITSLISQAAQLSTQAQPSNQSPMSLTSDASSPRSYVSPRISTPQTNTVPIKPLISTPPVSSQPKVSTPVVKQGPVSQSATQQPVTADKQQGHEPVSPRSLQRSSSQRSPSPGPNHTSNSSNASNATVVPQNSSARSTCSLTPALAAHFSENLIKHVQGWPADHAEKQASRLREEAHNMGTIHMSEICTELKNLRSLVRVCEIQATLREQRILFLRQQIKELEKLKSEFLHGVKM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WW domain containing adaptor with coiled-coil |
| 别名 | bA48B24, BM 016, DESSH, KIAA1844, PRO1741 |
| 计算分子量 | 647 aa, 65 kDa |
| 观测分子量 | 75-90 kDa |
| GenBank蛋白编号 | BC004258 |
| 基因名称 | WAC |
| Gene ID (NCBI) | 51322 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BTA9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WAC is an adaptor protein involved in gene transcription, protein ubiquitination, and autophagy. WAC links the RNA polymerase II transcriptional machinery with the histone H2B monoubiquitination machinery at lysine 120 (H2BK120ub1). It achieves this by using its WW domain to interact with RNA polymerase II and its coiled-coil domain to interact with the RNF20-RNF40 complex, thus coordinating transcriptional output. (PMID: 37402055, PMID: 40653822)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for WAC antibody 20519-1-AP | Download protocol |
| IP protocol for WAC antibody 20519-1-AP | Download protocol |
| WB protocol for WAC antibody 20519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
