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验证数据展示

  • WB analysis using 87005-3-RR (same clone as 87005-3-PBS)

    WB analysis using 87005-3-RR (same clone as 87005-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87005-3-RR (Vitronectin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87005-3-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 87005-3-RR (same clone as 87005-3-PBS)

    IP experiment of human plasma using 87005-3-RR (same clone as 87005-3-PBS)

    IP result of anti-Vitronectin/S-protein (IP:87005-3-RR, 4ug; Detection:87005-3-RR 1:1000) with human plasma lysate 3200 ug. This data was developed using the same antibody clone with 87005-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02822-2

    Sandwich ELISA sample test of MP02822-2

    Plasma of eight individual healthy human donors was measured. The Vitronectin/S-protein concentration of detected samples was determined to be 165.81 μg/mL with a range of 132.65-226.01 μg/mL.

  • Sandwich ELISA standard curve of MP02822-2

    Sandwich ELISA standard curve of MP02822-2

    Sandwich ELISA standard curve of MP02822-2, Human Vitronectin/S-protein Recombinant Matched Antibody Pair - PBS only. 87005-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4682. 87005-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

Vitronectin/S-protein Recombinant monoclonal antibody, PBS Only (Capture)

Vitronectin/S-protein Uni-rAb® Recombinant Antibody for WB, IP, Sandwich ELISA, Indirect ELISA
Cat No. 87005-3-PBS

产品说明书

CloneNo. 252166E4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IP, Sandwich ELISA, Indirect ELISA

别名

Serum-spreading factor, Somatomedin-B, S-protein, V75, Vitronectin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VTN Ready-To-Use IHC Kit. VTN primary antibody included.

产品信息

87005-3-PBS targets Vitronectin/S-protein as part of a matched antibody pair:

MP02822-2: 87005-3-PBS capture and 87005-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IP, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Eg4682

Product name: Recombinant Human Vitronectin protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-478 aa of NM_000638.4

Sequence: DQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vitronectin
别名 Serum-spreading factor, Somatomedin-B, S-protein, V75, Vitronectin
计算分子量54 kDa
观测分子量65-75 kDa
GenBank蛋白编号NM_000638.4
基因名称 VTN
Gene ID (NCBI) 7448
RRIDAB_3745268
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04004
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

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