Anti-SARS-CoV-2 S-RBD protein Human IgM ELISA Kit
Range
1.56-100 ng/mL
Validation Data Gallery
Product Information
KE30004 is a qualitative measurement of the human IgM for 2019-nCoV S-RBD in serum and plasma. The principle of the kit is indirect ELISA. S-RBD Protein (HEK293 cells expression) has been pre-coated onto microplate well. The samples are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated anti-Human IgM is added to each well. Producing an complex "Recombinant Protein-human anti-S-RBD IgM antibody-HRP conjugated second antibody". after incubation the wells are washed, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Anti-SARS-CoV-2 S-RBD protein Human IgM ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Indirect |
| Sensitivity | N/A |
| Range | 1.56-100 ng/mL |
| Reactivity | |
| Tested applications | Indirect ELISA |
| Gene ID (NCBI) | 43740568 |
Background Information
A promising target for both diagnosis and therapeutics treatments of the new disease named COVID-19 is the coronavirus (CoV) spike (S) glycoprotein. The spike protein, which is responsible for the "corona" (Latin word for crown) appearance in all coronaviruses, is a type I glycoprotein that has an especial role in the interaction between the virus and the host cell. This protein attaches itself to specific cellular receptors and suffers a conformational change that enables the fusion of the virus and the cell. Studies have shown that the SARS-CoV-2's S RBD protein interacts strongly with the Angiotensin-converting enzyme 2 (ACE2). S-RBD protein in order to enlighten the binding epitopes of these Abs. Because of the conservation of S-RBD protein sequence and its strong immunogenicity, the S-RBD protein of coronavirus is chosen as a diagnostic tool. COVID-19 antibodies can be produced by a host immune system following exposure to SARS-CoV-2. IgM and IgG antibodies are also known as immunoglobulins M and G, respectively, and are among the antibody isotypes produced by vertebrate immune systems. The ELISA microplate is coated with the SARS-CoV-2 S-RBD protein. The coated S-RBD protein binds with COVID-19 IgM S-RBD antibodies in the serum sample.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | COVID-19 RBD, RBD, Spike, spike protein, S protein |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Infection SARS-CoV-2 nucleocapsid protein, rather than spike protein, triggers a cytokine storm originating from lung epithelial cells in patients with COVID-19 | ||
Nutrients Dynamic Changes in Antibodies and Proteome in Breast Milk of Mothers Infected with Wild-Type SARS-CoV-2 and Omicron: A Longitudinal Study | ||
Vaccines (Basel) Longitudinal Analyses after COVID-19 Recovery or Prolonged Infection Reveal Unique Immunological Signatures after Repeated Vaccinations | ||
Cell Rep PD-1(high)CXCR5(-)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection | ||
J Med Virol Pre-existing immunity to SARS-CoV-2 associates with strong T cell responses induced by inactivated COVID-19 vaccines | ||
PLoS One RBD-specific antibody response after two doses of different SARS-CoV-2 vaccines during the mass vaccination campaign in Mongolia |
