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验证数据展示

  • IHC staining of human colon using 86978-2-RR (same clone as 86978-2-PBS)

    IHC staining of human colon using 86978-2-RR (same clone as 86978-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86978-2-RR (VIL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86978-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included
  • IHC staining of human colon using 86978-2-RR (same clone as 86978-2-PBS)

    IHC staining of human colon using 86978-2-RR (same clone as 86978-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86978-2-RR (VIL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86978-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included
  • IF Staining of human small intestine using 86978-2-RR (same clone as 86978-2-PBS)

    IF Staining of human small intestine using 86978-2-RR (same clone as 86978-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human small intestine tissue using VIL1 antibody (86978-2-RR, Clone: 251823A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86978-2-PBS in a different storage buffer formulation.

  • IF Staining of human small intestine using 86978-2-RR (same clone as 86978-2-PBS)

    IF Staining of human small intestine using 86978-2-RR (same clone as 86978-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human small intestine tissue using VIL1 antibody (86978-2-RR, Clone: 251823A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86978-2-PBS in a different storage buffer formulation.

Villin Recombinant monoclonal antibody, PBS Only

Villin Uni-rAb® Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 86978-2-PBS

产品说明书

CloneNo. 251823A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

VIL1, D2S1471, VIL, villin 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.

产品信息

86978-2-PBS targets Villin in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9610

Product name: Recombinant human VIL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC017303

Sequence: MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 villin 1
别名 VIL1, D2S1471, VIL, villin 1
计算分子量827aa,93 kDa; 826aa,93 kDa
GenBank蛋白编号BC017303
基因名称 Villin
Gene ID (NCBI) 7429
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09327
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

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