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验证数据展示

  • WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

    IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included
  • IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included

Villin Monoclonal antibody, PBS Only

Villin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66096-1-PBS

产品说明书

CloneNo. 2B7B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

VIL1, 2B7B9, D2S1471, VIL, villin 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.

产品信息

66096-1-PBS targets Villin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9637

Product name: Recombinant human VIL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC017303

Sequence: MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 villin 1
别名 VIL1, 2B7B9, D2S1471, VIL, villin 1
计算分子量827aa,93 kDa; 826aa,93 kDa
观测分子量 93-95 kDa
GenBank蛋白编号BC017303
基因名称 Villin
Gene ID (NCBI) 7429
RRIDAB_2881495
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09327
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

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