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验证数据展示

  • IHC staining of mouse lung using 82864-7-RR

    IHC staining of mouse lung using 82864-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 82864-7-RR

    IHC staining of mouse lung using 82864-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 82864-7-RR

    IF Staining of NIH/3T3 using 82864-7-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Vegfa antibody (82864-7-RR, Clone: 2I18 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82864-7-RR

    Affinity and Kinetic Characterization of 82864-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82864-7-RR against Mouse Vegfa were performed. The affinity constant is below 1 pM.

VEGFA Recombinant antibody

VEGFA Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 82864-7-RR

产品说明书

CloneNo. 2I18

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF/ICC, ELISA

别名

2I18, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse lung tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82864-7-RR targets VEGFA in IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 VEGFA fusion protein Eg32035 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular endothelial growth factor A
别名 2I18, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A
GenBank蛋白编号NM_001287056
基因名称 Vegfa
Gene ID (NCBI) 22339
RRIDAB_3670586
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

实验方案

Product Specific Protocols
IHC protocol for VEGFA antibody 82864-7-RRDownload protocol
IF protocol for VEGFA antibody 82864-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols