验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human heart tissue, human kidney tissue, human lung tissue, MCF-7 cells, mouse brain tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IF | See 3 publications below |
产品信息
12354-1-AP targets VTI1A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | VTI1A fusion protein Ag3018 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vesicle transport through interaction with t-SNAREs homolog 1A (yeast) |
| 别名 | Vti1-rp2, Vesicle transport v-SNARE protein Vti1-like 2, Vesicle transport through interaction with t-SNAREs homolog 1A |
| 计算分子量 | 217 aa, 25 kDa |
| 观测分子量 | 25-29 kDa |
| GenBank蛋白编号 | BC017052 |
| 基因名称 | VTI1A |
| Gene ID (NCBI) | 143187 |
| RRID | AB_2216150 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AJ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). VTI1A is involved in transport between the endosome and the trans-Golgi network. The SNARE complex of STX10, STX16, VTI1A, and VAMP3 is required for MPR (mannose 6-phosphate receptors) transport from endosomes to the Golgi after delivering lysosomal enzymes to the endocytic pathway (PMID: 18195106). Transport from early/recycling endosomes to the TGN is mediated by the SNARE complex of STX6, STX16, VTI1A and VAMP4 (PMID: 11839770; 21807881).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VTI1A antibody 12354-1-AP | Download protocol |
| IF protocol for VTI1A antibody 12354-1-AP | Download protocol |
| IP protocol for VTI1A antibody 12354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport. | ||
J Cell Sci The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes. | ||
Oncotarget Downregulation of vimentin expression increased drug resistance in ovarian cancer cells. | ||
Traffic Deficiency of the Cog8 subunit in normal and CDG-derived cells impairs the assembly of the COG and Golgi SNARE complexes. | ||
Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. |
