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B7‑H4 Monoclonal antibody

B7‑H4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E5B1

Cat No : 66817-1-Ig

Print datasheet

Synonyms

B7 H4, B7h.5, B7H4, B7‑H4, B7S1, B7X, FLJ22418, PRO1291, Protein B7S1, RP11 229A19.4, VCTN1, VTCN1

验证数据展示

  • WB analysis using 66817-1-Ig

    WB analysis using 66817-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66817-1-Ig (B7‑H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 66817-1-Ig

    IHC staining of rat kidney using 66817-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VTCN1 antibody included

经过测试的应用

Positive WB detected inSKOV-3 cells, T-47D cells, HeLa cells, A549 cells
Positive IHC detected inrat kidney tissue, human cervical cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VTCN1 Ready-To-Use IHC Kit. VTCN1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66817-1-Ig targets B7‑H4 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen B7‑H4 fusion protein Ag27751 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name V-set domain containing T cell activation inhibitor 1
Synonyms B7 H4, B7h.5, B7H4, B7‑H4, B7S1, B7X, FLJ22418, PRO1291, Protein B7S1, RP11 229A19.4, VCTN1, VTCN1
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 31-35 kDa
GenBank Accession NumberBC065717
Gene Symbol VTCN1
Gene ID (NCBI) 79679
RRIDAB_2882160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7Z7D3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

实验方案

Product Specific Protocols
WB protocol for B7‑H4 antibody 66817-1-IgDownload protocol
IHC protocol for B7‑H4 antibody 66817-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols