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VTA1 Polyclonal antibody

VTA1 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15786-1-AP

Print datasheet

Synonyms

C6orf55, DRG 1, DRG1, HSPC228, LIP5, LYST interacting protein 5, My012, SBP1, SKD1 binding protein 1, VTA1

验证数据展示

  • WB analysis using 15786-1-AP

    WB analysis using 15786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15786-1-AP

    IP experiment of HEK-293 using 15786-1-AP

    IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:300) with HEK-293 cells lysate 3000 ug.

  • IF Staining of HeLa using 15786-1-AP

    IF Staining of HeLa using 15786-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, human urine sample, rat brain tissue, fetal human brain tissue
Positive IP detected inHEK-293 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15786-1-AP targets VTA1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen VTA1 fusion protein Ag8485 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Vps20-associated 1 homolog (S. cerevisiae)
Synonyms C6orf55, DRG 1, DRG1, HSPC228, LIP5, LYST interacting protein 5, My012, SBP1, SKD1 binding protein 1, VTA1
Calculated Molecular Weight 307 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC006989
Gene Symbol VTA1
Gene ID (NCBI) 51534
RRIDAB_2288524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VTA1 antibody 15786-1-APDownload protocol
IF protocol for VTA1 antibody 15786-1-APDownload protocol
IP protocol for VTA1 antibody 15786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomic analyses uncover a new function and mode of action for mDia2.

Authors - Tadamoto Isogai
View Article