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VSX2 Polyclonal antibody

VSX2 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25825-1-AP

Print datasheet

Synonyms

CHX10, Homeobox protein CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, VSX2

验证数据展示

  • IF Staining of mouse eye using 25825-1-AP

    IF Staining of mouse eye using 25825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse eye using 25825-1-AP

    WB analysis of mouse eye using 25825-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 25825-1-AP (VSX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human retinoblastoma using 25825-1-AP

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human retinoblastoma using 25825-1-AP

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman retinoblastoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse eye tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

25825-1-AP targets VSX2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen VSX2 fusion protein Ag23035 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name visual system homeobox 2
Synonyms CHX10, Homeobox protein CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, VSX2
Calculated Molecular Weight 361 aa, 39 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC128153
Gene Symbol VSX2
Gene ID (NCBI) 338917
RRIDAB_2880257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VSX2 antibody 25825-1-APDownload protocol
IHC protocol for VSX2 antibody 25825-1-APDownload protocol
IF protocol for VSX2 antibody 25825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Human brain organoids assemble functionally integrated bilateral optic vesicles.

Authors - Elke Gabriel
View Article
humanIF

Nat Protoc

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Authors - Elke Gabriel
View Article