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VILIP-1 Polyclonal antibody

VILIP-1 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13919-1-AP

Print datasheet

Synonyms

Hippocalcin like protein 3, HLP3, HPCAL3, HUVISL1, VILIP, VILIP 1, VILIP1, VILIP-1, visinin like 1, Visinin like protein 1, VISL1, VLP 1, VLP1, VLP-1, VSNL1

验证数据展示

  • WB analysis using 13919-1-AP

    WB analysis using 13919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 13919-1-AP

    IP experiment of mouse brain using 13919-1-AP

    IP result of anti-VILIP-1 (IP:13919-1-AP, 3ug; Detection:13919-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

  • IF Staining of HeLa using 13919-1-AP

    IF Staining of HeLa using 13919-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13919-1-AP (VILIP-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse tongue tissue, human brain tissue, rat brain tissue, HeLa cells, mouse cerebellum tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13919-1-AP targets VILIP-1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen VILIP-1 fusion protein Ag4923 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name visinin-like 1
Synonyms Hippocalcin like protein 3, HLP3, HPCAL3, HUVISL1, VILIP, VILIP 1, VILIP1, VILIP-1, visinin like 1, Visinin like protein 1, VISL1, VLP 1, VLP1, VLP-1, VSNL1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-24 kDa
GenBank Accession NumberBC022012
Gene Symbol VSNL1
Gene ID (NCBI) 7447
RRIDAB_2215851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VILIP-1 antibody 13919-1-APDownload protocol
IF protocol for VILIP-1 antibody 13919-1-APDownload protocol
IP protocol for VILIP-1 antibody 13919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB,IF

Free Radic Biol Med

Dimerization of visinin-like-protein 1 is regulated by oxidative stress and calcium and is a pathological hallmark of amyotrophic lateral sclerosis.

Authors - Martina P Liebl
View Article
mouseWB

Front Cell Neurosci

Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.

Authors - Anna Sancho-Balsells
View Article