验证数据展示
经过测试的应用
| Positive IHC detected in | mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21056-1-AP targets VSIG2 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | VSIG2 fusion protein Ag14342 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | V-set and immunoglobulin domain containing 2 |
| 别名 | 2210413P10Rik, CT like protein, CTH, CTXL, VSIG2 |
| 计算分子量 | 327 aa, 34 kDa |
| GenBank蛋白编号 | BC007313 |
| 基因名称 | VSIG2 |
| Gene ID (NCBI) | 23584 |
| RRID | AB_3669354 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96IQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VSIG2, also known as CTXL (cortical thymocyte-like protein), belongs to the V-Set and immunoglobulin (Ig) domain-containing (VSIG) family. The VSIG family is also a member of the immunoglobulin superfamily (IgSF). VSIG2 has been recognized as a novel immune checkpoint that plays a role in regulating immune response. VSIG2 was associated with the development and progression of colon adenocarcinoma (COAD) and pancreatic ductal adenocarcinoma (PDAC) and could be used as a potential biomarker(PMID: 34290531;PMID: 37626304).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for VSIG2 antibody 21056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |