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VSIG1 Polyclonal antibody

VSIG1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14145-1-AP

Print datasheet

Synonyms

1700062D20Rik, Cell surface A33 antigen, Glycoprotein A34, GPA34, VSIG1

验证数据展示

  • WB analysis of human stomach using 14145-1-AP

    WB analysis of human stomach using 14145-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14145-1-AP (VSIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 14145-1-AP

    IHC staining of human testis using 14145-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14145-1-AP (VSIG1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 14145-1-AP

    IHC staining of human testis using 14145-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14145-1-AP (VSIG1 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman stomach tissue
Positive IHC detected inhuman testis tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14145-1-AP targets VSIG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen VSIG1 fusion protein Ag5314 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name V-set and immunoglobulin domain containing 1
Synonyms 1700062D20Rik, Cell surface A33 antigen, Glycoprotein A34, GPA34, VSIG1
Calculated Molecular Weight 387 aa, 42 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC043216
Gene Symbol VSIG1
Gene ID (NCBI) 340547
RRIDAB_2272980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XK7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VSIG1 antibody 14145-1-APDownload protocol
IHC protocol for VSIG1 antibody 14145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

Downregulation of cancer-associated fibroblast exosome-derived miR-29b-1-5p restrains vasculogenic mimicry and apoptosis while accelerating migration and invasion of gastric cancer cells via immunoglobulin domain-containing 1/zonula occluden-1 axis

Authors - Chenqu Wu
View Article