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验证数据展示

  • IHC staining of human breast cancer using 15608-1-AP

    IHC staining of human breast cancer using 15608-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15608-1-AP (VRK3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15608-1-AP

    IHC staining of human breast cancer using 15608-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15608-1-AP (VRK3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15608-1-AP

    WB analysis using 15608-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15608-1-AP (VRK3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

VRK3 Polyclonal antibody

VRK3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15608-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

vaccinia related kinase 3, VRK3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15608-1-AP targets VRK3 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 VRK3 fusion protein Ag7993 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vaccinia related kinase 3
别名 vaccinia related kinase 3, VRK3
计算分子量 53 kDa
观测分子量 50-53 kDa
GenBank蛋白编号BC023556
基因名称 VRK3
Gene ID (NCBI) 51231
RRIDAB_10863120
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IV63
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VRK3 antibody 15608-1-APDownload protocol
IHC protocol for VRK3 antibody 15608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols