验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human liver tissue, mouse skeletal muscle tissue, K-562 cells, U2OS cells, BxPC-3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
12946-1-AP targets VRK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | VRK2 fusion protein Ag4019 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vaccinia related kinase 2 |
| 别名 | Vaccinia-related kinase 2, vaccinia related kinase 2, Serine/threonine-protein kinase VRK2, EC:2.7.11.1 |
| 计算分子量 | 508 aa, 58 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC027854 |
| 基因名称 | VRK2 |
| Gene ID (NCBI) | 7444 |
| RRID | AB_2215681 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86Y07 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vaccinia-related kinase 2 (VRK2) is a serine/threonine kinase that plays a significant role in various cellular processes, including cell survival, proliferation, and DNA damage response. The VRK2 gene is known to produce two main splice variants: VRK2A and VRK2B, with VRK2A being the predominant form in humans. VRK2 is involved in several key biological functions. It is known to enhance cell survival by acting as an anti-apoptotic factor, which is particularly crucial in cancer biology. The overexpression of VRK2 has been linked to increased drug sensitivity in cancer cells, suggesting that this kinase may play a role in therapeutic responses. Additionally, high levels of VRK2 protein have been associated with improved survival rates in specific subgroups of astrocytomas, a type of brain tumor. (PMID: 29872222; 37943248; 24079673)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VRK2 antibody 12946-1-AP | Download protocol |
| IHC protocol for VRK2 antibody 12946-1-AP | Download protocol |
| IP protocol for VRK2 antibody 12946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. | ||
Int J Biol Sci VRK2 activates TNFα/NF-κB signaling by phosphorylating IKKβ in pancreatic cancer. | ||
BMC Med Reduced Vrk2 expression is associated with higher risk of depression in humans and mediates depressive-like behaviors in mice
| ||
Cell Rep Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice
|
