验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-85598-2 targets VRK1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag27754 Product name: Recombinant human VRK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-396 aa of BC103761 Sequence: EDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDLSVVENGGLKAKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTRKRVQK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | vaccinia related kinase 1 |
| 别名 | EC:2.7.11.1, Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1 |
| 计算分子量 | 396 aa, 45 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC103761 |
| 基因名称 | VRK1 |
| Gene ID (NCBI) | 7443 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99986 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vaccinia-related kinase 1 (VRK1) is a members of the vaccinia-related kinase (VRK) family of serine/threonine kinases and are principally localized in the nucleus. VRK1 was initially described as a kinase with homology to B1R, a vaccinia virus kinase required for viral DNA replication and the early phase of infection. It has been suggested that VRK1 was overexpressed in numerous human tumors, such as lung carcinomas, hepatocellular carcinoma, breast carcinomas, head and neck squamous cell carcinoma. (PMID: 28927264, PMID: 29103766)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VRK1 antibody CL488-85598-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
